The aim of this study was to develop a fast, simple and accurate analytical method for the classification of reference tablet analgesic drugs containing dipyrone, orphenadrine and caffeine using differential scanning calorimetry (DSC) and visible-near infrared spectroscopy (VNIRS) associated with one-class chemometric classification algorithm. The training set is based on reference medicine with 15 samples as target class. Three different brands with 10 samples each and five reference medicine samples, obtaining 35 samples, were used as the test set. Chemometric models based on principal component analysis (PCA) and data-driven soft independent modelling of class analogy (DD-SIMCA) were used to obtain the results. Two DD-SIMCA models obtained 100% sensitivity, specificity, and accuracy using DSC and VNIRS, both with a significance level of 0.01. This method using one-class classification as a chemometric tool proved to be a good alternative for quality control of pharmaceutical samples.

Abstract Dental caries and periodontal disease are the most prevalent of the biofilm-dependent diseases. With numerous side effects on the use of chlorhexidine, the search for new safe therapeutic alternatives for microorganisms involved with these diseases increases every day. This study aimed to evaluate the antimicrobial activity and cytotoxicity of extracts made from the bark of Schinopsis brasiliensis Engl. against five oral microorganisms and analyze their phytochemical and thermal degradation profile. The liquid-liquid partition was performed with hexane, chloroform and ethyl acetate. The identification and quantification of the chemical marker was done. Antimicrobial activity was evaluated based on the minimum inhibitory concentration. The cytotoxicity was analyzed based on the hemolysing potential of the samples. The thermal degradation profile was performed by two different methods. Gallic acid was identified as the main compound of the samples and showed the highest amount in the chloroform fraction. All samples were able to inhibit the growth of the microorganisms tested and showed no cytotoxicity. The ethanol extract absorbs less heat than the fractions. All samples exhibited exothermic peak consistent with degradation of gallic acid. Based on the results, the samples used are potential candidates for use in dental formulations for biofilm control.